Designing and testing PCRs (RT-PCR) to quickly test people for COVID19 Infection
Global health crises have been caused by the COVID 19 epidemic. A quick detection method is crucial to stop its spread. A PCR (RTPCR) test must be developed to detect the COVID19 original strain as well as the newer variants, Delta and Omicron. The protocol is in place and it’s now up to you to identify the correct primers for each of the three strains. To identify the correct primers it’s important to classify variants according to the type of mutations acquired. This is usually a missense, point, or nonsense mutation. (Larson and al., 2019). After that, the PCR primers need to be targeted at the particular mutations within the variants while being specifically for COVID19 (Tsai et.al., 2020). Primers must target, for example, Omicron or Delta variants of the spike protein gene. However, they should also be specific to COVID19 viruses. Cont….