Which enzymes are involved in the Ub mediated proteolysis pathway? The Role of Enzymes in Ubiquitin-Mediated Proteolysis Pathway

Ubiquitin-Mediated Proteineolysis Pathway: The Role Of Enzymes

Ubiquitin mediated proteolysis (UBM) is an essential process where proteins are marked with ubiquitin before being routed to the proteaseasome for their degradation. The enzymes involved in this process act in a sequence to make sure that proteins can be efficiently degraded. Three major classes of enzymes are involved in this process, including E1, E2, and E3 ubiquitin-conjugating enzymes. E1 enzyme activates and binds ubiquitin in an ATP molecule. This is the initial step. The E1 enzyme activates ubiquitin, and then transfers it to the E2 enzyme. This catalyzes the formation an isopeptide bonded between activatedubiquitins and target proteins. A final step involves an E3 enzyme. This facilitates the interaction of the ubiquitin with the target protein and thus completes the process. These enzymes are crucial in Ubiquitin-mediated proteolysis. Recent research has shown that mutations and deficiencies of E1, E2, or E3 enzymes can be linked to numerous diseases and disorders. (Brot et. al. 2016; Chen, 2021). These enzymes are crucial in the Ubiquitin-mediated proteolysis process. Further research on their function may be useful to help understand different diseases. Cont…