The principle behind the Next Generation Sequencing (NGS)

The precept behind the Subsequent Era Sequencing (NGS)

The precept behind the Subsequent Era Sequencing (NGS) methodology of Illumina Sequencing is to make use of the precept of bridge amplification to generate quick DNA fragments (150-300 base pairs) that are then sequenced (Mallick et al., 2017). The method begins by ligation of quick fragments of DNA to adapters, that are then separated into particular person clusters and added to a stream cell. These DNA fragments are then amplified to create hundreds of thousands of copies of the unique fragments. The fragments are then sequenced by including fluorescently labeled nucleotides that may be detected and recorded by the Illumina sequencer (Singh et al., 2020). The sequencing course of is repeated hundreds of thousands of occasions, producing hundreds of thousands of reads that are then aligned to a reference genome to generate a sequence. The top result’s a high-quality, huge quantity of information which can be utilized for quite a lot of purposes resembling variant detection, gene expression evaluation, and microbial identification (DeJesus et al., 2019).