The polymerase chain reaction (PCR) has three major steps: 1. Denaturation using high temperature 2. Annealing using DNA primer 3. Extension using nucleotides and taq polymerase (an enzyme very similar to DNA Polymerase III) a) Describe how each of the three steps stated above are similar and/or different from DNA replication in eukaryotic cells. Exploring the Similarities and Differences between PCR and DNA Replication in Eukaryotic Cells

Exploring the Similarities and Variations between PCR and DNA Replication in Eukaryotic Cells

The method of polymerase chain response (PCR) and DNA replication in eukaryotic cells share a number of similarities, nonetheless these processes additionally exhibit many variations. One of many main commonalities between the 2 processes is using DNA primers. In each PCR and DNA replication, the primers are used to indicate the start and finish of the template DNA strand to be replicated (O’Reilly et al., 2017). Step one of PCR is the denaturation of the DNA utilizing excessive temperatures. That is in distinction to DNA replication in eukaryotic cells, which doesn’t require excessive temperatures to separate the 2 strands of the template DNA (Federico et al., 2020). The second step of PCR is the annealing of the primers to the DNA strands, which can also be just like the method of replication in eukaryotic cells (Liu et al., 2016). The ultimate step of PCR is the extension of the primer utilizing nucleotides and the enzyme Taq Polymerase, which is analogous to using DNA Polymerase III in eukaryotic cells. Nevertheless, the distinction within the enzymes used for the completion of the replication course of is the primary distinction between PCR and DNA replication in eukaryotic cells (Gao et al., 2021). Cont….

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