If you were interested in identifying an unknown protein in a sample, what approach would you use? Identifying an Unknown Protein in a Sample: An Overview

Unknown protein in sample: A review

Identification of unknown proteins in samples is an intricate process that requires expertise and care. There are many approaches that can be employed depending on how complex the sample is and what the goal is. The steps include determining the amount of protein in the sample, altering it to allow for analysis and analysing its chemical and structural characteristics. Mass spectrometry (MS), which is commonly used to quantify an unidentified protein in a sample, is the best (Frenz et. al. 2016,). The technique is useful for obtaining detailed information on the molecular structure and functions of the protein. To determine the amount of the protein, other techniques, such as chromatography and enzyme-linked immunesorbent assay, can be used (Amara et. al., 2018,). The sample can now be tested for structural and chemical properties after it has been prepared. You can use techniques like circular dichroism and nuclear magnetic resonance (NMR), depending on what you want (Viel and al., 2019,). The detailed three-dimensional structure can also be determined using X-ray crystallography (Krause and al., 2002). Cont…