Strategies and enzymes used in the Cloning of Genes
Gene Cloning refers to the process of obtaining a specific genetic copy by cloning a gene. This is done using enzymes that cut and paste DNA fragments. Then they are inserted in the correct organism. Cloning genes can be done in several ways, such as polymerase chain reactions (PCR), restriction fragment length polymorphisms (RFLP), or recombinant genetic technology. A method that creates a collection of DNA fragments is called restriction fragment length polymorphism. The restriction enzymes are used to reduce the gene of concern. Once the fragments have been separated, they can be used to make a gene map. The polymerase-chain reaction (PCR), is a technique that amplifies a single copy DNA to create thousands. To copy the gene of your choice, you will need a thermostable enzyme such as Taq polymerase. It is used to synthesize the complementary strand, which creates multiple copies. The combination of DNA from various sources using recombinant technology is one method. This involves using enzymes such as DNA Ligase to connect DNA from various sources. After the DNA is linked it can be transferred to Escherichia coli, which will then allow it to become a host organism. Cont….