Analyse of the Intracellular Distributions of Giardia IscU Proteins and Human Proteins By Cell Fractionation
Cell fractionation refers to a method that separates cells based on their size and density. The intracellular distributions for the Giardia protein and human proteins have been analysed using this technique. The cell homogenate was then centrifuged at low speed in order to separate the pellet (low-speed sediment), from the supernatant of low speed. To separate the supernatant (high-speed sediment) and the cytosol, the supernatant (low-speed sediment) was spun at high speed. This study has helped us to gain a better understanding of the intracellular distribution of these proteins (Kumar, et. al. 2021, Yi, et. al. 2016, etc). The pellet is made up of organelles such as the nucleus and mitochondria. However, the supernatant is mainly composed of cytoplasmic elements in solution. High-speed centrifugation results in a pellet containing mainly membrane bound organelles. The supernatant, however, contains mostly cytosolic compounds. Results of this analysis revealed that Giardia IscU’s main location is in the cell cytosol and human protein in the membrane bound organelles.